MicroTally® as a validated sampling method

Official Guidance using MicroTally® products:

1. FSIS Directive:

Sampling Verification Activities for Shiga Toxin-Producing Escherichia coli in Raw Beef Products.

  • Analytes: Seven adulterant STEC serogroups (E. coli O157, O26, O45, O103, O111, O121, and O145) and Salmonella
  • Matrixes: Beef manufacturing trimmings and bench trim.

2. Beef Industry Food Safety Council:

Guidance Document for Lotting and Sampling of Beef Products for Pathogen Analysis

  • Analytes: Salmonella and the 6 specific serotypes (O26, O103, O45, O111, O121 and O145) of shiga toxin-producing E. coli that are regulated as adulterants in beef
  • Matrixes: Beef products.

PCR assays validated with sampling cloth:

1. BioControl Systems, Inc./MilliporeSigma

Assurance® GDS EHEC ID for E. coli O157:H7 Tq

  • Analytes: E. coli O157:H7, E. coli O157: NM
  • Matrixes: Raw ground beef, raw beef trim, frozen finely textured beef, carcass cloths.

2. Bio-Rad Laboratories:

dd-Check STEC Solution

  • Analytes: Shiga-toxin producing E. coli (STEC)
  • Matrixes: Raw beef trim (375 g and 325 g), fresh spinach (375 g), carcass sampling cloths.

iQ-Check STEC VirX & iQ-Check STEC SerO

  • Analytes: STEC VirX: shiga toxin-producing Escherichia coli (STEC), Stx1, Stx2, eae; STEC Sero: E. coli O26, O45, O103, O111, O121, O145, O157:H7
  • Matrixes: Raw beef trim, ground beef, fresh spinach, beef carcass sampling cloth (4″ x 4″ MicroTally® cloth), cannabis flower, dried hemp flower, all-purpose flour. Cannabis infused gummies, cannabis infused chocolate, and cannabis derived concentrates.

3. BioMérieux, SA:

GENE-UP® Pathogenic E. coli (PEC) V2

  • Analytes: Shiga-toxin producing E. coli (STEC) containing highly correlated co-localized virulence genes stx and eae
  • Matrixes: Raw ground beef, raw beef trim, Romaine lettuce, and carcass sampling cloth (MicroTally® or equivalent) from beef trim.

GENE-UP® QUANT Salmonella V6

  • Analytes: Salmonella species
  • Matrixes: Raw ground turkey, chicken carcass rinsate, raw beef trim, carcass sampling cloth (MicroTally® or equivalent) quantitation of Salmonella as low as 1 CFU/g in breaded and stuffed raw chicken.

GENE-UP® QUANT Salmonella 2 (SLM 2)

  • Analytes: Salmonella species
  • Matrixes: Liquid whole egg, cooked ham, raw ground beef, raw chicken, lettuce, dry pet food, chocolate, stainless steel sponge, raw beef, eggs, chicken carcass rinse, raw ground chicken, raw beef trim, raw ground pork, raw ground bison, pasteurized liquid egg, powered egg, whey protein powder, creamy peanut butter, dark chocolate, garlic powder, milk chocolate, whole cannabis flower, whole hemp flower, carcass sampling cloth for beef and pork (MicroTally® or equivalent), boot swabs

4. Eurofins

Eurofins GeneScan BACGene Mplex STEC Screen Kit

  • Analytes: Shiga-toxin producing E. coli (STEC)
  • Matrixes: Fresh raw beef trim.

5. Gold Standard Diagnostics

BACGene E. coli O157:H7 workflow

  • Analytes: Escherichia. coli O157:H7
  • Matrixes: Fresh raw ground beef, fresh raw beef trim, beef carcass sampling cloth, Romaine lettuce, all-purpose flour, stainless steel, unglazed ceramic tile.

BACGene STEC TOP 7 workflow

  • Analytes: STEC TOP 7 (E. coli O157:H7, O26, O45, O103, O111, O121, O145)
  • Matrixes: Fresh raw ground beef, fresh raw beef trim, beef carcass sampling cloth, Romaine lettuce, all-purpose flour, stainless steel, unglazed ceramic tile.

6. Hygiena

BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact

  • Analytes: E. coli O157:H7
  • Matrixes: Fresh raw ground beef, fresh raw beef trim, leafy greens, raw fluid milk, dried cannabis flower & dried hemp flower, sampling cloths swabbed from 375 g beef trim portions.

BAX® System Real-Time PCR Assay for Salmonella

  • Analytes: Salmonella
  • Matrixes: Raw ground beef, chicken carcass rinse, bagged lettuce, cream cheese, dry pet food, stainless steel, milk chocolate, chocolate liquor, cocoa powder, shell egg, plastic, raw comminuted chicken, turkey, whole carcass poultry rinses, fresh raw beef trim, MicroTally® on fresh raw beef trim, fresh raw ground pork, fresh raw pork trim, MicroTally® on fresh raw pork trim, dried cannabis flower, dried hemp flower, sampling cloths swabbed from 375 g beef trim portions.

BAX® System Real-Time PCR Assay Suite for STEC

  • Analytes: stx, eae virulence genes, E. coli O26, O45, O103, O111, O121, O145
  • Matrixes: Raw beef trim, raw ground beef, raw ground beef plus soy, all-purpose flour, dried cannabis flower & dried hemp flower, Sampling cloths swabbed from 375 g beef trim portions.

Food Safety in the Beef Industry

  • Analytes: Escherichia coli O157:H7, STEC and Salmonella
  • Matrixes: Ground beef and beef trim.

Quantification and Limits Manual

  • Analytes: Salmonella spp., Campylobacter spp., Genus Listeria, Listeria monocytogenes, E. coli O157:H7 and Vibrio spp.
  • Matrixes: Raw ground beef, chicken carcass rinse, bagged lettuce, cream cheese, dry pet food, stainless steel, milk chocolate, chocolate liquor, cocoa powder, shell egg, plastic, raw comminuted chicken, turkey, whole carcass poultry rinses, fresh raw beef trim, MicroTally® on fresh raw beef trim, fresh raw ground pork, fresh raw pork trim, MicroTally® on fresh raw pork trim, dried cannabis flower, dried hemp flower, sampling cloths swabbed from 375 g beef trim portions. 

Enrichment validated with sampling cloth

1. Pathotrak, Inc.:

Pathotrak Next Generation Enrichment

  • Analytes: Salmonella spp. & Shiga-toxin producing Escherichia coli (STEC)
  • Matrixes: Romaine hearts 375g & 1,500 g, beef trim sampling cloths.