Testing Method Matrix Extension Validation

PCR assays validated with sampling cloth:

Analytes: E. coli O157:H7, E. coli O157: NM
Matrixes: Raw ground beef, raw beef trim, frozen finely textured beef, carcass cloths.

Analytes: Shiga-toxin producing E. coli (STEC)
Matrixes: Raw beef trim (375 g and 325 g), fresh spinach (375 g), carcass sampling cloths.

Analytes: STEC VirX: shiga toxin-producing Escherichia coli (STEC), Stx1, Stx2, eae; STEC Sero: E. coli O26, O45, O103, O111, O121, O145, O157:H7
Matrixes: Raw beef trim, ground beef, fresh spinach, beef carcass sampling cloth (4″ x 4″ MicroTally® cloth), cannabis flower, dried hemp flower, all-purpose flour. Cannabis infused gummies, cannabis infused chocolate, and cannabis derived concentrates.

Analytes: Shiga-toxin producing E. coli (STEC) containing highly correlated co-localized virulence genes stx and eae
Matrixes: Raw ground beef, raw beef trim, Romaine lettuce, and carcass sampling cloth (MicroTally® or equivalent) from beef trim.

Analytes: Salmonella species
Matrixes: Raw ground turkey, chicken carcass rinsate, raw beef trim, carcass sampling cloth (MicroTally® or equivalent) quantitation of Salmonella as low as 1 CFU/g in breaded and stuffed raw chicken.

Analytes: Escherichia. coli O157:H7
Matrixes: Fresh raw ground beef, fresh raw beef trim, beef carcass sampling cloth, Romaine lettuce, all-purpose flour, stainless steel, unglazed ceramic tile.

Analytes: STEC TOP 7 (E. coli O157:H7, O26, O45, O103, O111, O121, O145)
Matrixes: Fresh raw ground beef, fresh raw beef trim, beef carcass sampling cloth, Romaine lettuce, all-purpose flour, stainless steel, unglazed ceramic tile.

Analytes: E. coli O157:H7
Matrixes: Fresh raw ground beef, fresh raw beef trim, leafy greens, raw fluid milk, dried cannabis flower & dried hemp flower, sampling cloths swabbed from 375 g beef trim portions.

Analytes: Salmonella
Matrixes: Raw ground beef, chicken carcass rinse, bagged lettuce, cream cheese, dry pet food, stainless steel, milk chocolate, chocolate liquor, cocoa powder, shell egg, plastic, raw comminuted chicken, turkey, whole carcass poultry rinses, fresh raw beef trim, MicroTally® on fresh raw beef trim, fresh raw ground pork, fresh raw pork trim, MicroTally® on fresh raw pork trim, dried cannabis flower, dried hemp flower, sampling cloths swabbed from 375 g beef trim portions.

Analytes: stx, eae virulence genes, E. coli O26, O45, O103, O111, O121, O145
Matrixes: Raw beef trim, raw ground beef, raw ground beef plus soy, all-purpose flour, dried cannabis flower & dried hemp flower, Sampling cloths swabbed from 375 g beef trim portions.

Analytes: Salmonella spp., Campylobacter spp., Genus Listeria, Listeria monocytogenes, E. coli O157:H7 and Vibrio spp.
Matrixes: Raw ground beef, chicken carcass rinse, bagged lettuce, cream cheese, dry pet food, stainless steel, milk chocolate, chocolate liquor, cocoa powder, shell egg, plastic, raw comminuted chicken, turkey, whole carcass poultry rinses, fresh raw beef trim, MicroTally® on fresh raw beef trim, fresh raw ground pork, fresh raw pork trim, MicroTally® on fresh raw pork trim, dried cannabis flower, dried hemp flower, sampling cloths swabbed from 375 g beef trim portions.

Analytes: Escherichia coli genes, stx1 and/or stx2, eae
Matrixes: Fresh raw beef trim, fresh raw ground beef, fresh raw ground pork, sampling cloth from raw beef, sampling cloth from raw pork, sampling mitt from raw chicken parts, sampling mitt from raw turkey carcass, fresh poultry parts, mechanically separated chicken, fresh spinach, sprouts, dried cannabis flower, dried hemp flower.